TitleDetermination of parent and substituted polycyclic aromatic hydrocarbons in high-fat salmon using a modified QuEChERS extraction, dispersive SPE and GC-MS.
Publication TypeJournal Article
Year of Publication2011
AuthorsForsberg ND, Wilson GR, Anderson KA
JournalJ Agric Food Chem
Date Published08/2011
Animals, Fats, Food Contamination, Gas Chromatography-Mass Spectrometry, Polycyclic Hydrocarbons, Aromatic, Salmon, Seafood, Solid Phase Extraction

A fast and easy modified QuEChERS (quick, easy, cheap, rugged and safe) extraction method has been developed and validated for determination of 33 parent and substituted polycyclic aromatic hydrocarbons (PAHs) in high-fat smoked salmon that greatly enhances analyte recovery compared to traditional QuEChERS procedures. Sample processing includes extraction of PAHs into a solution of ethyl acetate, acetone and isooctane followed by cleanup with dispersive SPE and analysis by GC-MS in SIM mode. Method performance was assessed in spike recovery experiments (500 μg/g wet weight) in three commercially available smoked salmon with 3-11% fat. Recoveries of some 2-, 3- and 5-ring PAHs were improved 50-200% over traditional methods, while average recovery across all PAHs was improved 67%. Method precision was good with replicate extractions typically yielding relative standard deviations <10%, and detection limits were in the low ng/g range. With this method, a single analyst could extract and clean up ≥60 samples for PAH analysis in an 8 h work day.

Alternate JournalJ. Agric. Food Chem.
PubMed ID21732651
PubMed Central IDPMC3237295
Grant ListP42 ES016465 / ES / NIEHS NIH HHS / United States
P42 ES016465-02 / ES / NIEHS NIH HHS / United States
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