TitleChemical marker for ALS-inhibitor herbicides: 2-aminobutyric acid proportional in sub-lethal applications.
Publication TypeJournal Article
Year of Publication2002
AuthorsLoper BR, Cobb WT, Anderson KA
JournalJ Agric Food Chem
Date Published2002 Apr 24
Acetolactate Synthase, Aminobutyric Acids, Autoanalysis, Chromatography, Liquid, Enzyme Inhibitors, Herbicides, Kinetics, o-Phthalaldehyde, Solanum tuberosum, Trichloroacetic Acid

A chemical profiling technique for sub-lethal acetolactate synthase (ALS)-inhibitor herbicides (e.g., sulfonylureas, imidazolines, triazolopyrimidine sulfonanilides, and pyrimidyloxy salicylic) was developed using 2-aminobutyric acid, and was found to be directly proportional to application rates in field studies on two varieties of potato plants. An uncomplicated, benign-by-design analytical method for the determination of 2-aminobutyric acid in plant tissue was developed. The method is simple, fast, and automated, entailing a water-trichloroacetic acid extraction followed by precolumn on-line derivatization using o-phthalaldehyde (OPA) solution and liquid chromatographic analyses. Use of reagents and chlorinated organic solvents, and generation of waste, are minimized as compared to other ALS-inhibitor herbicide analytical techniques. Recoveries for a series of fortified plant tissues ranged from 82 to 103%. Two 20-day field trials on two potato varieties, Russet Burbank and Shepody, were conducted during the 2000 and 2001 growing seasons. The study demonstrated that the 2-aminobutyric acid method is an excellent, selective chemical marker technique for ALS-inhibitor herbicides for real world plant matrixes.

Alternate JournalJ. Agric. Food Chem.
PubMed ID11958629